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KMID : 0377220080330040020
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Medical Journal of Chosun Univercity
2008 Volume.33 No. 4 p.20 ~ p.25
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Regulation of Vibrio vulnificus Hemolysin Production by Changes of Temperature and Salinity
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Shin Sung-Hee
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Abstract
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Vibrio vulnificus can survive two distinct envi-ronments: sea water habitats and the human body. Entering the human body, V. vulnificus must be able to modulate virulence expression for survival by sensing the changes of many environmental factors including temperature and salinity. In this study, we tested whether the shifting of temperature (25 to 37oC) and salinity (2.5 to 0.9%) modulates the production of the potent exotoxin hemolysin (VvhA) via the ToxRS signal transduction system. In a merozygotic PvvhA:: lacZ transcriptional reporter, vvhA tran-scription and VvhA production levels were higher in 0.9% and at 37oC than in 2.5% and at 25oC. VvhA production was decreased by toxR mutation and this decreased VvhA production was almost completely rescued by in trans-complementation of wild-type toxR. However, VvhA production was still modulated by the temperature and salinity shifts in the toxR-mutated background. These results suggest that V. vulnificus senses the changes of temperature and salinity to modulate VvhA production, probably via yet-unknown systems other than the ToxRS system
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KEYWORD
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Vibrio vulnificus, Hemolysin, ToxRS sys-tem, Temperature, Salinity
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